Team:IISER TVM/parts basic
Parts
Basic Parts
Bacterial Chitinase Combo 1 ( BBa_K3979004)
This is a recombinant chitinase gene designed using the catalytic domain(CD) of ChiB from Pseudoalteromonas sp. DL-6 and the chitin-binding domain (CBD) of ChiB (Uniprot ID: P11797) from Serratia marcescens. CD-CBD forms the basic chitinase gene framework. The protein sequence is codon-optimized for E. coli expression. BamHI and HindIII sites were added at the 5’ and 3’ end of the gene, respectively.
Bacterial Chitinase Combo 2 (BBa_K3979003)
This is a recombinant chitinase gene designed using the catalytic domain(CD) of chitinase from Amycolatopsis orientalis and the chitin-binding domain (CBD) of ChiB from Serratia marcescens. CD-CBD forms the basic chitinase gene framework. The protein sequence is codon-optimized for E. coli expression. BamHI and HindIII sites were added at the 5’ and 3’ end of the gene, respectively.
Plant Chitinase Combo 1 (BBa_K3979007)
This is a recombinant chitinase gene designed using the catalytic domain (CD) of Triticum aestivum cultivar Sumai 3 and the chitin-binding domain (CBD) of Hordeum vulgare cultivar NK1558. The sequences of both barley and wheat chitinases are joined using common (GGGS)3 linkers. Barley CBD is added to the C-terminal of the combination. The protein sequence is codon-optimized for E. coli expression. BamHI and HindIII sites were added at the 5’ and 3’ end of the gene, respectively.
Plant Chitinase Combo 2 (BBa_K3979008)
This is a recombinant chitinase gene designed using the catalytic domain (CD) of Triticum aestivum cultivar Sumai 3 and the chitin-binding domain (CBD) of Hordeum vulgare cultivar NK1558. The chimeric chitinase consists of a wheat catalytic domain flanked by two barley chitin-binding domains. The protein sequence is codon-optimized for E. coli expression. BamHI and HindIII sites were added at the 5’ and 3’ end of the gene, respectively.
Chitinase B from Serratia marcescens QMB1466 (BBa_K3979009)
Chitinase B from Serratia marcescens QMB1466 is a hydrolyzing enzyme that can hydrolyze insoluble chitin to its oligo and monomeric components: N-Acetylglucosamine. The molecular size and weight of the protein are 55.46438 kDa and 1524 base pairs. The protein sequence is codon-optimized for E. coli expression. BamHI and HindIII sites were added at the 5’ and 3’ end of the gene, respectively.
ChiA from Amycolatopsis orientalis (BBa_K3979010)
Chitinase A (ChiA) from Amycolatopsis orientalis strain B-37 is a hydrolyzing enzyme that can hydrolyze insoluble chitin to its oligo and monomeric components: N-Acetylglucosamine. The molecular size and weight of the protein are 33.13 kDa and 975 base pairs. The protein sequence is codon-optimized for E. coli expression. BamHI and HindIII sites were added at the 5’ and 3’ end of the gene, respectively.
Chitinase II precursor from Triticum aestivum (BBa_K3979005)
Chitinase II precursor derived from Triticum aestivum cultivar Sumai 3 is a hydrolyzing enzyme that can hydrolyze insoluble chitin to its oligo and monomeric components: N-Acetylglucosamine. The molecular size and weight of the protein are 24.73 kDa and 719 base pairs. The protein sequence is codon-optimized for E. coli expression. BamHI and HindIII sites were added at the 5’ and 3’ end of the gene, respectively.
Endochitinase 1 from Hordeum vulgare (BBa_K379006)
Endochitinase 1 derived from Hordeum vulgare cultivar NK1558 is a hydrolyzing enzyme that can hydrolyze insoluble chitin to its oligo and monomeric components: N-Acetylglucosamine. The molecular size and weight of the protein are 33.40 kDa and 981 base pairs. The protein sequence is codon-optimized for E. coli expression. BamHI and HindIII sites were added at the 5’ and 3’ end of the gene, respectively.
